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pH to drop from pH 82 to 74 This time was long because charge transfer led to buildup of a potential difference across the membrane (Donnan potential), which was slow to dissipate The time was dropped to about 300 ms in the resence of a K ionophore, valinomycin, an antiporter type of effect The proton ionophore, bis(hexa uoroacetonyl)acetone, dropped the reequilibration time down to <1 ms Discussions of the possible mechanisms of H transport ensued It was pointed out that the solubility of water in n-alkanes was high enough to suggest the participation of membrane-dissolved water in the transport mechanism Biegel and Gould [409] predicted that the SUVs used in their study could have 30 40 H2O molecules dissolved in the bilayer hydrocarbon (HC) core Meier et al [411] measured the concentration of water in the HC interior of bilayes to be about 100 mM Two short reviews discussed proton conductance: Nagle [412] defended the position that water wires inside the HC core can explain H conductance; Gutknecht [413] questioned that view, proposing that fatty acid impurities can also explain the phenomenon, in a ip- op movement of the neutralized weak acid Proton carriers such as CO2 or H2CO3 could also be involved [415] The last word has not been said on this topic Using liposomes made from phospholipids as models of membrane barriers, Chakrabarti and Deamer [417] characterized the permeabilities of several amino acids and simple ions Phosphate, sodium and potassium ions displayed effective permeabilities 01 1:0 10 12 cm/s Hydrophilic amino acids permeated membranes with coef cients 51 5:7 10 12 cm/s More lipophilic amino acids indicated values of 250 410 10 12 cm/s The investigators proposed that the extremely low permeability rates observed for the polar molecules must be controlled by bilayer uctuations and transient defects, rather than normal partitioning behavior and Born energy barriers More recently, similar magnitude values of permeabilities were measured for a series of enkephalin peptides [418].

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members are accessible both from static and non-static methods, using their simple names No NullPointerException is thrown, as ref refers to an instance of MyClass 422 (c) Local variables can have the same name as member variables The local variables will simply shadow the member variables with the same names Declaration (4) defines a static method that tries to access a variable named a, which is not locally declared Since he method is static, this access will only be valid if variable a is declared static within the class Therefore, declarations (1) and (4) cannot occur in the same class declaration, while declarations (2) and (4) can 423 (b) The keyword this can only be used in instance (non-static) methods Only one occurrence of each static variable of a class is created, when the class is loaded by the JVM This occurrence is shared among all the objects of the class (and for that matter, by other clients) Local variables are only accessible within the block scope, regardless of whether the block scope is defined within a static context 424 (c) A class can be declared abstract even if it does not delcare any abstract methods The variable k cannot be declared synchronized Only methods and blocks can be synchronized 425 (c) The declaration in (c) is not legal, as variables cannot be declared abstract The keywords static and final are valid modifiers for both field and method declarations The modifiers abstract and native are valid for methods, but not for fields 426 (a) and (c) Abstract classes can declare both final methods and non-abstract methods Nonabstract classes cannot, however, contain abstract methods Nor can abstract classes be final Only methods can be declared native 427 (a) The keyword transient signifies that the fields should not be stored when objects are serialized Constructors cannot be declared abstract When an array object is created, as in (c), the elements in the array object are assigned the default value corresponding to the type of the elements Whether the reference variable denoting the array object is a local or a member variable is irrelevant Abstract methods from a superclass need not be implemented by a subclass, but the subclass must then be declared abstract.

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